Sybr Green Master Mix

Sybr green i dye is a double stranded dna binding dye that detects any double stranded dna generated during pcr.

Sybr green master mix.

3 x 1 7 ml 2x quantifast sybr green pcr master mix contains rox dye 2 x 2 ml rnase free water. This mastermix is suited for use with self designed qpcr assays on other types of real time pcr instruments with fast cycling conditions. Sybr green realtime pcr master mix plus is effective for reducing non specific amplifications or primer dimers in the intercalation assay. Many pcr machines require a passive reference dye.

Sybr green realtime pcr master mix is applicable for intercalation assay with sybr green i. The master mixes contain buffer dntps thermostable hot start dna polymerase and of course sybr green dye everything needed for real time pcr except the sample and pcr primer pair. Powerup sybr green master mix features include. Rt 2 preamp cdna synthesis kit 12 rt 2 nano preamp cdna synthesis kit.

Coupled with user supplied primer sets and template powerup sybr green master mix is designed to amplifiy targets for accurate gene expression analysis. The master mixes contain buffer dntps thermostable hot start dna polymerase and of course sybr green dye everything needed for real time pcr except the sample and pcr primer pair. A single cdna synthesis reaction is sufficient to interrogate hundreds of mirnas. Rt 2 sybr green fast mastermixes are available with rox fluorescein or without reference dyes.

Power sybr green pcr master mix delivers superior sensitivity and reproducibility without compromising any other performance parameters such as specificity wide dynamic range and uniformity. Applied biosystems sybr green pcr master mix provides maximum flexibility at reduced cost because no target specific taqman probes are required. Sybr green master mixes are available with other dyes including rox. An internal reference dye such as rox corrects well to well optical variations and is used for fluorescent signal normalization.

In addition power sybr green pcr master mix provides high fluorescent signal strength for consistent detection of low copy numbers. Rt 2 sybr green fast mastermixes are specially formulated for use with the 100 ring disc format of the rt 2 profiler and rt 2 lncrna pcr arrays on the rotor gene q. Applied biosystems sybr green master mixes are designed for quantitative real time pcr using a set of two pcr primers that flank the target region. Sybr green master mixes are 2x concentrated and contain dntps mgcl 2 and dna polymerase.

Powerup sybr green master mix is a pre formulated optimized universal 2x master mix for real time pcr workflows. The miscript sybr green pcr kit includes quantitect sybr green pcr master mix and the miscript universal primer which is specific for the universal tag sequence added to the 5 end of the cdna during reverse transcription.