Sybr Green Pcr Master Mix

The lightcycler 480 sybr green i master is a one component hot start reaction mix for pcr.

Sybr green pcr master mix.

The master mixes contain buffer dntps thermostable hot start dna polymerase and of course sybr green dye everything needed for real time pcr except the sample and pcr primer pair. The sybr green pcr master mix is a convenient premix of the components except primers template and water necessary to perform real time pcr using sybr green i dye. Sybr green i dye is a double stranded dna binding dye that detects any double stranded dna generated during pcr. The master mixes contain buffer dntps thermostable hot start dna polymerase and of course sybr green dye everything needed for real time pcr except the sample and pcr primer pair.

For 200 x 50 µl reactions. It contains faststart taq dna polymerase and dna double strand specific sybr green i dye for product detection and characterization. Power sybr green pcr master mix contains a proprietary version of applied biosystems rox dye an internal passive reference to normalize non pcr related fluorescence fluctuations. 3 x 1 7 ml 2x quantitect sybr green pcr master mix 100 µl ung solution 2 x 2 ml rnase free water.

Sybr green realtime pcr master mix plus is effective for reducing non specific amplifications or primer dimers in the intercalation assay. Applied biosystems sybr green master mixes are designed for quantitative real time pcr using a set of two pcr primers that flank the target region. Itaq universal sybr green supermix is a 2x concentrated ready to use reaction master mix optimized for dye based quantitative pcr qpcr on any real time pcr instrument. Applied biosystems sybr green pcr master mix provides maximum flexibility at reduced cost because no target specific taqman probes are required.

Sybr green realtime pcr master mix is applicable for intercalation assay with sybr green i. Applied biosystems sybr green master mixes are designed for quantitative real time pcr using a set of two pcr primers that flank the target region.